Investigations into Enzymatic Bioconversion to Form Rebaudioside A from Stevioside
نویسندگان
چکیده
منابع مشابه
Bioconversion of rebaudioside I from rebaudioside A.
To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy (1H, 13C, COSY, HSQC-DEPT,...
متن کاملSynthesis of rebaudioside A from stevioside and their interaction model with hTAS2R4 bitter taste receptor.
Steviol glycosides (SG's) from Stevia rebaudiana (Bertoni) have been used as a natural low-calorie sweeteners. Its aftertaste bitterness restricts its use for human consumption and limits its application in food and pharmaceutical products. In present study, we have performed computational analysis in order to investigate the interaction of two major constituents of SG's against homology model ...
متن کاملA Rapid Dialysis Method for Analysis of Stevioside and Rebaudioside A in Foods.
A rapid dialysis method for the analysis of stevioside (SS) and rebaudioside A (RS) in foods was developed. Minced samples (10 g) were packed into 30 cm net length dialysis tubing with 30% methanol to increase the dialysis efficiency. The dialysis tubing was put in a 100 mL centrifuge tube, and the total fluid volume was made up to 100 mL with 30% methanol. Dialysis was done with shaking while ...
متن کاملHPTLC method for simultaneous analysis of stevioside and rebaudioside-A in Stevia rebaudiana.
An HPTLC method was developed and validated for simultaneous determination of stevioside (STE) and rebaudioside-A (REB-A) in Stevia rebaudiana leaves. The HPTLC separation was performed on precoated silica gel 60F254 HPTLC plates with the mobile phase ethyl acetate-ethanol-acetone-water (15 + 3 + 6 + 6, v/v/v/v). The densitometric quantification of steviol glycosides was carried out at Amax 580...
متن کاملPharmacokinetics of rebaudioside A and stevioside after single oral doses in healthy men.
This randomized, double-blind, cross-over study assessed the comparative pharmacokinetics of steviol and steviol glucuronide following single oral doses of rebaudioside A and stevioside in healthy adult male subjects. Steviol glucuronide appeared in the plasma of all subjects after administration of rebaudioside A or stevioside, with median tmax values of 12.0 and 8.00h post-dose, respectively....
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ژورنال
عنوان ژورنال: Periodica Polytechnica Chemical Engineering
سال: 2018
ISSN: 1587-3765,0324-5853
DOI: 10.3311/ppch.12673